UJI AKTIVITAS PREVENTIF ANTIDIABETES DAUN KOPASANDA (CHROMOLAENA ODORATA L.) DALAM UPAYA PEMBUATAN SERBUK TEH CELUP

Soufa Malita; Muhammad Junaedi

doi:10.31004/prepotif.v9i2.46519

Authors

Soufa Malita Prodi Teknologi Pangan, Institut Teknologi dan Kesehatan Aspirasi
Muhammad Junaedi Prodi Administrasi Kesehatan, Institut Teknologi dan Kesehatan Aspirasi

DOI:

https://doi.org/10.31004/prepotif.v9i2.46519

Keywords:

Kopasanda; Chromolaena Odorata L.; Preventif; Antidiabetes; Aloksan

Abstract

Kopasanda (Chromolaena Odorata L.) merupakan tanaman gulma yang tumbuh subur di seluruh wilayah Indonesia. Bagian daun kopasanda mengandung senyawa metabolit yang memberi pengaruh secara signifikan terhadap diabetes. Daun kopasanda dalam penelitian ini dibuat dalam bentuk serbuk teh celup dan dilakukan uji aktivitas preventif untuk mengetahui daya hambatnya terhadap penyakit diabetes. Tujuan penelitian adalah untuk mengetahui aktivitas preventif teh daun kopasanda dalam menghambat munculnya penyakit diabetes yang banyak diderita masyarakat. Metode penelitian di mulai dari pembuatan serbuk teh celup daun kopasanda dan dilanjutkan dengan melakukan uji aktivitas preventif antidiabetes pada hewan uji tikus dengan diinduksi aloksan. Sebanyak 36 ekor tikus putih betina, dibagi dalam 6 kelompok (6 ekor/ kelompok) yaitu kontrol normal (tanpa induksi aloksan), kontrol (-) (induksi aloksan), kontrol (+) (induksi aloksan dan metformin), kelompok perlakuan dosis I (PLI: 40mg/200gBB), kelompok perlakuan dosis II (PLII: 80mg/200gBB) dan kelompok perlakuan dosis III (PLIII: 120mg/200gBB). Sampel diberikan mulai dari 2 minggu sebelum induksi aloksan sampai akhir perlakuan. Parameter yang diamati adalah kadar glukosa darah pada setiap hewan uji. Hasil menunjukkan bahwa kadar glukosa darah pada PL I turun sebanyak 28%, PL II turun 25% dan PL III turun 17%. Hasil uji statistik dengan uji ANOVA menunjukkan bahwa kelompok perlakuan signifikan lebih rendah dibandingkan kontrol (-) dan kontrol (+) (P<0.05). Uji Bonferroni kelompok kontrol (-) menunjukkan perbedaan secara signifikan terhadap kelompok Normal, Kontrol (+), serta PL I, II dan III (P<0.05). Berdasarkan hasil penelitian diketahui bahwa teh dari daun kopasanda dapat menurunkan kadar glukosa darah dengan baik. Dosis 40mg/200gBB merupakan hasil terbaik terhadap penurunan kadar glukosa darah. Teh daun kopasanda memiliki aktivitas preventif sebagai antidiabetes.

References

Abdulkadir, A., Nafisat, O. A., Sakariyau, A. W., Maimuna, B. U., Fatimah, M. M., Yahaya, M. A. (2023). Inhibitory Effects of Moringa Oleifera Seed Extracts on Crude Xanthine Oxidase. GSC Biological and Pharmaceutical Sciences. Vol 23, no 3, p.120-128.

Aini, F., Hardani., En, P., Musparlin, H. (2022). Antibacterial Activity Test of Kopasanda Leaf Extract (Chromolaena odorata L.) Against Bacteria Staphylococus epidermidis. Pharmaceutical & Traditional Medicine. Vol 6. No 1, p. 1-7.

Anugrahini, C. P. H., & Arifah, S. W. (2021). Narrative Review: Antidiabetic Activity of Traditional Plants in Java Island. Pharmacon: Jurnal Farmasi Indonesia. E-ISSN 2685-5062, p. 120-131.

Arbilla, A. H., Inas, L. C., Firyal, F. (2023). Herbal Plants to Reduce Blood Glucose in Diabetes Mellitus Patients. Nautical: Jurnal Ilmiah Multidisiplin. Vol 2, no 3, p. 192-195.

Djohari, M., Husnawati., Fina, A., Belia, S. B. (2023). The Effect of Giving Katuk Leaf Infusion (Sauropus androgynus (L.) Merr) on Blood Glucose Levels of Male White Mice (Mus Musculus L.) Induced by Alloxan. Jurnal Penelitian Farmasi Indonesia. Vol 12, no 1, p. 1-6.

Elokofehinti, O. O., Nicholas, A. A., Opeyemi, I. (2021). Antidiabetic Potential of Chromolaena Odorata Leave Extract And its Effect on Nrf2/Keap1 Antioxidant Pathway in The Liver of Diabetic-Induced Wistar Rats. Advances in Traditional Medicine. Vol 23, no 1, p. 513-523.

Fardha, A. M., Zainal, F., Yudha, A. J., Ari, K. (2023). Relationship Between Body Weight and Blood Sugar Levels in Diabetes Mellitus Screening Activities for the Elderly at the Alas Barat Health Center, Alas Barat District, Sumbawa Regency. Journal of Indonesia Laboratory Technology of Student. Vol 2, no 2, p. 1-9.

Hartati., Sahribulan., Nur, A. D. (2024). Potential of Kopasanda Leaf Ethanol Extract as Anti-Hyperglycemia. Jurnal Biologi Tropis. Vol 24, no 4, p.717-725.

Hasan, H., Endah, N. D., Faramita, H., Fika, N. R., Ichlasul, O. H. (2024). Phytochemical Screening and Antidiabetic Effect Test of Methanol Extract of Brotowali Leaves (Tinospora crispa L.) in Mencit (Mus musculus). Journal of Pharmacology and Natural Products. Vol 1, no 1, p. 20-32.

Lubis, A. A., Yunus, M., Naldi, J., Andry, M., Ginting, P., Safitri, F., Nasution. (2023). Antihyperuricemia Activity Test of Kopasanda Leaf Extract (Chromolaena Odorata (L.) R.M.King &; H.Rob against male white mice (Mus Musculus L.) induced potassium oxonate. Journal of Pharmaceutical and Sciences. Vol 6, no 3, hal 1273–1281.https://doi.org /10.36490/journal-jps.com.v6i3.209.

Marianne, Dwi, L. P., Elin, Y. S, Neng, F.K., Rosnani, N. (2014). Antidiabetic Activity of Leaves Ethanol Extract Chromolaena odorata (L.) R.M. King on Induced Male Mice with Alloxan Monohydrate. Jurnal Natural vol. 14, no 1, p. 1-4 (ISSN 1141-8513).

Maryam, S. t., Widyawati, Uni, A. P., Dian L. (2021). Kopasanda Leaves as an Alternative Plant to Counteract Free Radicals. Jurnal Kesehatan. Vol. 14, no 1. (E-ISSN (2622-7363) P-ISSN (2086-2555).

Munawwarah, Syamsul, B., Iwayan, M. (2021) The Effect of Kopasanda Leaves (Chromlaena Odorata) On The Speed Of Blood Clotting. Journal Pijar MIPA. Vol 16 no , hal :258-261. DOI: 10.29303/jpm.v16i2.2193.

Nangoy, B. N., Edwin, D. Q., Adithya, Y. (2019). Antidiabetic Activity Test of Sesewanua Leaf Extract (Clerodendron squamatum Vahl.) on Male Galu White Ratsr Wistar (Rattus norvegicus L.). Pharmacon, vol 8, no 4, p. 774.

Nurhajanah, M., Agussalim, L., Siti, Z. I., Titi, L. H. (2020). Analysis of Antiseptic Content of Kopasanda Leaves (Choromolaena Odorata) as a Basis for Making Gel on Wounds. Bioscientist: Scientific Journal of Biology. Vol 8, no 2, p. 284-293.

Nurhanifa, S. t., Ratnah, Sesilia, R. P. (2022). Antibacterial Potential of Kopasanda Leaf Extract (Chromolaena odorata L) Against Pseudomonas aeruginosa And Staphylococcus aureus. AKFARINDO. Vol. 7, no 2,p. 94-99.

Ojo, O. A., Hannah, S. I., Damilare, E. R., Akingbolabo, D. O., Adebola, B. O. (2023). Diabetes Mellitus: from Molecular Mechanism to Pathophysiology and Pharmacology. Medicine in Novel Technology and Devices. Vol. 19, p. 1-8.

Safna, F. L., Visi, K., Nurfadhillah, K., Moch.Erwin, R., Zulfiyah, S. (2021). The Role of Kersen Leaf Extract (Muntingia calabura L) on Changes in Blood Glucose Levels in Mice (Mus musculus). Fakumi Medical Journal. Vol 1, no 2, p. 88-96.

Shehadeh, M. B., Ghadeer, A. R. Y. S., Ala’, M. A. (2021) Plants Secondary Metabolites as Blood Glucose-Lowering Molecules. Molecules, vol. 26, no 14 doi: 10.3390/molecules26144333 PMCID: PMC8307461 PMID: 34299610.

Sukmawati, Aulia W.., Andi. M. S,. (2023). Test of the Effectiveness of Ethanol Extract of Kopasanda Leaves (Chromolaena odorata L.) on Epithelialization Ability in Rats (Rattus norvegicus) Induced by Burns. Journal Makassar Pharmaceutical Science. Vol 1, no 3, p. 13-18.

syaniyah, A. A., Iseu, S. A., Nisatami, H. (2024). Obesity Status and Blood Sugar Levels in Productive Age. Nutrition Scientific Journal. Vol 3, no 1, p. 44-51.

Tandra, H. (2021). Diabetics Can Eat Anything. Jakarta : Gramedia.

Tari, M, Dwi, I. (2021). Test of the Tonic Effect of Ethanol Extract of Kopasanda Leaves (Chromolaena odorata L.) on Male White Mice With Natatory Exhaustion Method. Journal Ilmiah Bakti Farmasi. Vol 5, no 1, p. 21-34.

Taufik, I.H. Diabetes mellitus in children. Ministry of Health Directorate General of Health Services. [Internet]. (2023). Available from: https://yankes.kemkes.go.id/view_artikel/2612/diabetes-melitus-in children.

Widiastuti & Slamet, R. (2024). Comparison of Blood Glucose Examination Methods Hexokinase and Peroxidase Methods in Wistar Rats (Rattus norvegicus). Journal of Islamic Pharmacy. Vol. 9, no 1, p. 27-30

Wirawan, A. Y., Rachmat, K., Herwin. (2023). Antibacterial Activity of Extra Ethanol Kopasanda Leaves (Chromolaena OdorateL.) Against Pathogenic Bacteria of Urinary Tract Infection by TLC-Bioautography and Agar Diffusion. Journal Microbiology Science. Vol. 3, no 2, p.10-19.

World Health Organization (WHO). Health Topics. [Internet]. (2024). Available from: https://www.who.int/health topics/diabetes?gad_source=1&gclid=CjwKC AjwnvvBh%20BdEiwABCYQA1xH4YBtnb_JrEYgucZh3Uoueur5RoKkTMNfKPLRx8IzcdIOzGBoCEtYQAvD%20BwE#tab=tab_1.

Wulandari, N. L. W. E., Udayani, N. N. W., Ni, L. K. A. A. D., Ginza, A. P. T., Ni, P. E. M. K. D., Ida, A. P. W., Anak, A. S. S. P. (2024). Artikel Review: The Effect of Alloxan Induction on Rat Blood Sugar. Indonesia Journal of Pharmaceutical Education. Vol. 2, no 2, p. 205-216.

Yasser, M, Ilham, N. M., Amri, Herman, B., Ninin, A., Ririn, U. S. (2022). Phytochemical Screening of Flavonoid, Alkaloid, Saponin, Steroid and Terpenoid Compounds from Kopasanda Leaves (Chromoloena odorata L.) Proceedings of the 6th National Seminar on Research & Community Service in the Field of Chemical Engineering, Analytical Chemistry, Environmental Engineering, Biochemistry and Bioprocess 90.

Yulianti, A., Ahmad, A. S., Uun, R. (2023). Effect of Moringa Leaf Flour Brewing on Fasting Blood Glucose Levels in Rats Diabetes Melitus. Jurnal Kesehatan. Vol. 11, no 1, p. 1-6.