PEMERIKSAAN DNA BAG1 TOXOPLASMA GONDII DALAM ORGAN HATI AYAM KAMPUNG DI PASAR DI SURABAYA

Authors

  • Irwin Prijatna Kusuma Medicine, School of Medicine, Universitas Ciputra, Surabaya
  • Hebert Adrianto Medicine, School of Medicine, Universitas Ciputra, Surabaya
  • Purwakaning Purnomo Agung RSUD Dr. Mohamad Soewandhie Surabaya
  • Victor Kurniawan Yuwono Medicine, School of Medicine, Universitas Ciputra, Surabaya
  • Saskia Dyah Handari Medicine, School of Medicine, Universitas Ciputra, Surabaya
  • Aldy Dion Medicine, School of Medicine, Universitas Ciputra, Surabaya
  • Astria Novitasari Nidom Professor Nidom Foundation Surabaya

DOI:

https://doi.org/10.31004/prepotif.v9i1.44155

Keywords:

ayam kampung, gen BAG1, hati, pasar, PCR

Abstract

Manusia dapat terinfeksi oleh T. gondii terutama melalui konsumsi daging mentah/setengah matang yang mengandung kista jaringan T. gondii atau melalui konsumsi makanan dan/atau air yang terkontaminasi ookista. Ayam yang terinfeksi T. gondii dapat berpotensi menularkan T. gondii ke manusia melalui konsumsi daging. Penelitian ini memiliki tujuan melakukan pemeriksaan DNA BAG1 T. gondii dalam organ hati ayam kampung yang diperoleh dari pasar di Surabaya Barat. Metode penelitian dilakukan secara obrservasional di laboratorium. Organ hati ayam kampung diambil dari beberapa pasar tradisional di Surabaya Barat secara kuota sampling. Organ hati dianalisis menggunakan Polymerase chain reaction (PCR) dengan gen BAG1. Panjang produk PCR gen BAG1 adalah 470 bp. Data penelitian dianalisis secara deskriptif dengan kategori kategori positif dan hasil negatif. Hasil pemeriksaan keberadaan parasit T. gondii dalam organ hati ayam kampung di Surabaya Barat melalui gen BAG1 adalah 50% sampel positif dan 50% sampel negatif. Perlu upaya skrining ayam yang dijual kepada konsumen dan penerapan PCR menggunakan gen BAG1 T. gondii dapat menjadi langkah awal untuk mencegah penyebaran infeksi toksoplasmosis kepada manusia.

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Published

2025-04-29

How to Cite

Kusuma, I. P., Adrianto, H., Agung, P. P., Yuwono, V. K., Handari, S. D., Dion, A., & Nidom, A. N. (2025). PEMERIKSAAN DNA BAG1 TOXOPLASMA GONDII DALAM ORGAN HATI AYAM KAMPUNG DI PASAR DI SURABAYA. PREPOTIF : JURNAL KESEHATAN MASYARAKAT, 9(1), 2272–2277. https://doi.org/10.31004/prepotif.v9i1.44155

Issue

Vol. 9 No. 1 (2025): APRIL 2025

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Articles

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Copyright (c) 2025 Irwin Prijatna Kusuma, Hebert Adrianto, Purwakaning Purnomo Agung, Victor Kurniawan Yuwono, Saskia Dyah Handari, Aldy Dion, Astria Novitasari Nidom

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