BERBERIN KUNYIT SEBAGAI REGULATOR MITOGENESIS DAN MITOFAGI DIABETES MELLITUS TIPE 2 DALAM PENCEGAHAN PENYAKIT INFEKSIUS PADA PESERTA IBADAH HAJI : STUDI IN SILICO
DOI:
https://doi.org/10.31004/prepotif.v9i1.43929Keywords:
Berberin Kunyit, Diabetes Mellitus Tipe 2, Sistem Imun, In SilicoAbstract
Diabetes Mellitus merupakan masalah kesehatan global dengan prevalensi, insidensi, dan mortalitas yang tinggi. Kondisi ini berdampak pada makroangiopati, mikroangiopati, serta gangguan sistem imun yang meningkatkan risiko infeksi. Indonesia, sebagai negara dengan jumlah Muslim terbesar, memiliki banyak peserta ibadah haji yang berisiko tinggi terhadap penyakit infeksi akibat diabetes. Hal ini berkontribusi pada peningkatan beban pengobatan individu maupun pemerintah. Zat aktif berberin dari kunyit (Fibraurea tinctoria lour) memiliki implikasi dalam penanganan disrupsi sistem imun akibat diabetes. Studi ini menggali potensi hubungan berberin dalam restorasi mitogenesis dan mitofagi melalui metode in silico dengan melakukan penambatan molekular menggunakan Autodock Tools. Hasil penambatan molekular menunjukkan ΔG binding rata-rata dengan PGC1a bernilai -6.79kJ/mol, sedangkan untuk berberine dan AMPK bernilai -7.68kJ/mol serta root mean square deviation (RMSD) tertinggi sebesar 0.95 untuk PGC1a dan 0,47 untuk AMPK. Studi in silico mengindikasikan berberin merupakan senyawa yang memiliki potensial untuk memodulasi kerja PGC1a dan AMPK melalui interaksi biokimianya yang energetically favorable dan akurat sehingga dapat merestorasi disregulasi sistem imun dalam menurunkan risiko infeksi pada peserta ibadah haji dengan diabetes mellitus tipe 2.References
Aulia, R., Maulana, H., Filio, Y. L., Shafira, N. A., Anindita, P. A., Suganda, T., Concibido, V., & Karuniawan, A. (2022). Assessment of rhizome yield of local Indonesian turmeric (Curcuma longa L.) during two growing seasons. Biodiversitas, 23(5), 2534–2543. https://doi.org/10.13057/biodiv/d230534
Bhansali, S., Bhansali, A., Walia, R., Saikia, U. N., & Dhawan, V. (2017). Alterations in Mitochondrial Oxidative Stress and Mitophagy in Subjects with Prediabetes and Type 2 Diabetes Mellitus. Frontiers in Endocrinology, 8. https://doi.org/10.3389/fendo.2017.00347
Bloemberg, D., & Quadrilatero, J. (2019). Autophagy, apoptosis, and mitochondria: molecular integration and physiological relevance in skeletal muscle. American Journal of Physiology-Cell Physiology, 317(1), C111–C130. https://doi.org/10.1152/ajpcell.00261.2018
Ding, W.-X., & Yin, X.-M. (2012). Mitophagy: mechanisms, pathophysiological roles, and analysis. Bchm, 393(7), 547–564. https://doi.org/10.1515/hsz-2012-0119
Ekins, S., Mestres, J., & Testa, B. (2007). In silico pharmacology for drug discovery: methods for virtual ligand screening and profiling. British Journal of Pharmacology, 152(1), 9–20. https://doi.org/10.1038/sj.bjp.0707305
Hang, W., He, B., Chen, J., Xia, L., Wen, B., Liang, T., Wang, X., Zhang, Q., Wu, Y., Chen, Q., & Chen, J. (2018). Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux. Frontiers in Pharmacology, 9. https://doi.org/10.3389/fphar.2018.01121
Kalfalah, F., Sobek, S., Bornholz, B., Götz-Rösch, C., Tigges, J., Fritsche, E., Krutmann, J., Köhrer, K., Deenen, R., Ohse, S., Boerries, M., Busch, H., & Boege, F. (2014). Inadequate mito-biogenesis in primary dermal fibroblasts from old humans is associated with impairment of PGC1A-independent stimulation. Experimental Gerontology, 56, 59–68. https://doi.org/10.1016/j.exger.2014.03.017
Karamanou, M. (2016). Milestones in the history of diabetes mellitus: The main contributors. World Journal of Diabetes, 7(1), 1. https://doi.org/10.4239/wjd.v7.i1.1
Khan, M. A. B., Hashim, M. J., King, J. K., Govender, R. D., Mustafa, H., & Al Kaabi, J. (2019). Epidemiology of Type 2 Diabetes – Global Burden of Disease and Forecasted Trends. Journal of Epidemiology and Global Health, 10(1), 107. https://doi.org/10.2991/jegh.k.191028.001
Kruse, R., Vind, B. F., Petersson, S. J., Kristensen, J. M., & Højlund, K. (2015). Markers of autophagy are adapted to hyperglycaemia in skeletal muscle in type 2 diabetes. Diabetologia, 58(9), 2087–2095. https://doi.org/10.1007/s00125-015-3654-0
Loebig, M., Klement, J., Schmoller, A., Betz, S., Heuck, N., Schweiger, U., Peters, A., Schultes, B., & Oltmanns, K. M. (2010). Evidence for a Relationship between VEGF and BMI Independent of Insulin Sensitivity by Glucose Clamp Procedure in a Homogenous Group Healthy Young Men. PLoS ONE, 5(9), e12610. https://doi.org/10.1371/journal.pone.0012610
Nair, A., & Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7(2), 27. https://doi.org/10.4103/0976-0105.177703
Papatheodorou, K., Banach, M., Bekiari, E., Rizzo, M., & Edmonds, M. (2018). Complications of Diabetes 2017. Journal of Diabetes Research, 2018, 1–4. https://doi.org/10.1155/2018/3086167
Reddy, S. S. K., & Tan, M. (2020). Diabetes mellitus and its many complications. In Diabetes Mellitus (pp. 1–18). Elsevier. https://doi.org/10.1016/B978-0-12-820605-8.00001-2
Regina, C. C., Mu’ti, A., & Fitriany, E. (2021). Systematic review tentang pengaruh obesitas terhadap kejadian komplikasi diabetes melitus tipe dua. Verdure: Health Science Journal, 3(1), 8–17.
Sasongko, M. B., Wardhana, F. S., Febryanto, G. A., Agni, A. N., Supanji, S., Indrayanti, S. R., Widayanti, T. W., Widyaputri, F., Widhasari, I. A., Lestari, Y. D., Adriono, G. A., Sovani, I., & Kartasasmita, A. S. (2020). The estimated healthcare cost of diabetic retinopathy in Indonesia and its projection for 2025. British Journal of Ophthalmology, 104(4), 487–492. https://doi.org/10.1136/bjophthalmol-2019-313997
Shan, Z., Fa, W. H., Tian, C. R., Yuan, C. S., & Jie, N. (2022). Mitophagy and mitochondrial dynamics in type 2 diabetes mellitus treatment. Aging, 14(6), 2902–2919. https://doi.org/10.18632/aging.203969
Statistica Research Department. (2021). Indonesia: Production of Turmeric 2021. Statistica Research Department. https://www.statista.com/statistics/706479/production-of-turmeric-in-indonesia/
United Nation. (2022). The 17 Goals. United Nation. https://sdgs.un.org/goals
Utami, R., Fernando, A., Sari, I. P., & Furi, M. (2017). Penetapan Kadar Berberin dari Ekstrak Etanol Akar dan Batang Sekunyit (Fibraurea Tinctoria Lour) dengan Metode KCKT. Jurnal Sains Farmasi & Klinis, 3(2), 115. https://doi.org/10.29208/jsfk.2017.3.2.84
WHO. (2022). Diabetes. World Health Organization. https://www.who.int/health-topics/diabetes
Ye, Y., Liu, X., Wu, N., Han, Y., Wang, J., Yu, Y., & Chen, Q. (2021). Efficacy and Safety of Berberine Alone for Several Metabolic Disorders: A Systematic Review and Meta-Analysis of Randomized Clinical Trials. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.653887
Yin, J., Ye, J., & Jia, W. (2012). Effects and mechanisms of berberine in diabetes treatment. Acta Pharmaceutica Sinica B, 2(4), 327–334. https://doi.org/10.1016/j.apsb.2012.06.003
Yu, Y. (2013). Pathogenesis of Diabetes mellitus DM), Type II. Calgary Guide. https://calgaryguide.ucalgary.ca/pathogenesis-of-diabetes-mellitus-dm-type-ii/dm-ii-pathogenesis/
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