EFEKTIVITAS KRIM EKSTRAK KULIT KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP  KEPADATAN KOLAGEN TIKUS PUTIH  JANTAN GALUR WISTAR YANG  DIPAPAR SINAR UV-B

Novina  Sari; Desi  Watri; Wenti Anggraeni

doi:10.31004/prepotif.v9i1.41182

Authors

Novina Sari Fakultas Kedokteran, Kedokteran Gigi dan Ilmu Kesehatan, Magister Sains Biomedis, Universitas Prima Indonesia
Desi Watri Fakultas Kedokteran, Kedokteran Gigi dan Ilmu Kesehatan, Magister Sains Biomedis, Universitas Prima Indonesia
Wenti Anggraeni Fakultas Kedokteran, Kedokteran Gigi dan Ilmu Kesehatan, Magister Sains Biomedis, Universitas Prima Indonesia

DOI:

https://doi.org/10.31004/prepotif.v9i1.41182

Keywords:

ekstrak, kayu manis, kolagen, tikus

Abstract

Penelitian ini bertujuan untuk menguji dan menganalisis efektivitas pemberian krim ekstrak kulit kayu manis (Cinnamomum burmannii) terhadap kepadatan kolagen tikus putih jantan galur wistar (Rattus norvegicus) yang dipapar sinar UVB. Penelitian yang digunakan pada penelitian ini yaitu kuantitatif eksperimental dengan menggunakan desain true experiment atau eksperimental laboratorium. Penelitian eksperimen dilaksanakan dengan mengontrol semua variabel luar yang dapat mempengaruhi kegiatan eksperimen. Penelitian ini menggunakan post-test only control group design untuk mengetahui dan menganalisis efek pemberian krim ekstrak kulit kayu manis dalam mempercepat pertumbuhan kolagen pada tikus putih (Rattus norvegicus) galur wistar yang dipapar sinar UVB. Hasil uji fitokimia menunjukkan bahwa ekstrak kulit kayu manis mengandung metabolit sekunder berupa flavonoid, saponin, alkaloid, tannin dan triterpenoid. Flavonoid berperan penting dalam membantu meningkatkan kepadatan kolagen. Hasil pengamatan kepadatan kolagen menunjukkan kelompok kontrol dan kelompok perlakuan memiliki perbedaan kepadatan kolagen yang signifikan. Kelompok kontrol yang hanya diolesi krim basis menghasilkan skor 1, yaitu kepadatan serabut kolagen kurang. Kelompok perlakuan 1 menghasilkan skor 2, yaitu kepadatan kolagen sedang. Kelompok perlakuan 2 dan 3 menghasilkan skor 3, yang maknanya kepadatan kolagen masuk dalam kategori rapat. Hasil pengamatan ekspresi melanin menunjukkan Kelompok perlakuan 3 yang diolesi krim ekstrak kulit kayu manis (Cinnamomum burmannii) dengan konsentrasi 12% mengalami penurunan ekspresi melanin yang lebih besar dibandingkan kelompok lainnya

References

American Burn Association. Burn Incidence and Treatment in the United States. 2016.Availablefrom: http://www.ameriburn.org/resources_factsheet.php [Accessed: August 15, 2024]

Ansary, T. M., Hossain, M. R., Kamiya, K., Komine, M., & Ohtsuki, M. (2021). Inflammatory molecules associated with ultraviolet radiation-mediated skin aging. International Journal of Molecular Sciences, 22(8), 3974.

Arda O, Göksügür N, Tüzün Y. Basic histological structure and functions of facial skin. Clin Dermatol. 2014;32:3–13.

Buttaro, T. M. (2019). ELECTRICAL INJURIES. Primary Care E-Book: Primary Care E-Book, 199.

Cavinato M, Waltenberger B, Baraldo G, Grade CVC, Stuppner H, Jansen-Dürr P. Plant extracts and natural compounds used against UVB-induced photoaging. Biogerontology. 2017 Aug;18(4):499-516. doi: 10.1007/s10522-017-9715-7. Epub 2017 Jul 12. PMID: 28702744; PMCID: PMC5514221.

Connolly M, Axtell A, Hickey S, Whalen A, McNamara L, Albright D, Friedstat J, Goverman J. Chemical Burn From Cinnamon Oil. Eplasty. 2017 Jun 1;17:ic11. PMID: 28607628; PMCID: PMC5459757.

D’Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013;14(6):12222–48. doi:10.3390/ ijms140612222

Dąbrowska, A. K., Spano, F., Derler, S., Adlhart, C., Spencer, N. D., & Rossi, R. M. (2018). The relationship between skin function, barrier properties, and body‐dependent factors. Skin Research and Technology, 24(2), 165-174.

Furukawa, J. Y., Martinez, R. M., Morocho-Jácome, A. L., Castillo-Gómez, T. S., Pereda-Contreras, V. J., Rosado, C., … Baby, A. R. (2021). Skin impacts from exposure to ultraviolet, visible, infrared, and artificial lights – a review. Journal of Cosmetic and Laser Therapy, 1–7. doi:10.1080/14764172.2021.1950767

Gu, Y., Han, J., Jiang, C., & Zhang, Y. (2020). Biomarkers, oxidative stress and autophagy in skin aging. Ageing research reviews, 59, 101036.

Greenhalgh, D. G. (2019). Management of burns. New England journal of medicine, 380(24), 2349-2359.

Gromkowska-Kępka KJ, Puścion-Jakubik A, Markiewicz-Żukowska R, Socha K. The impact of ultraviolet radiation on skin photoaging - review of in vitro studies. J Cosmet Dermatol. 2021 Nov;20(11):3427-3431. doi: 10.1111/jocd.14033. Epub 2021 Mar 13. PMID: 33655657; PMCID: PMC8597149.

González Maglio DH, Paz ML, Leoni J. Sunlight effects on immune system: is there something else in addition to UV-induced immunosuppression? Biomed Res Int. 2016;2016:1–10. doi: 10.1155/2016/1934518.

Holick MF.Biological effects of sunlight, ultraviolet radiation, visible light, infrared radiation and Vitamin D for health. Anticancer Res. 2016;36(3):1345–56.

Ishida-Yamamoto A, Igawa S, Kishibe M. Molecular basis of the skin barrier structures revealed by electron microscopy. Exp Dermatol. 2018:0–2. https://doi.org/10.1111/exd.13674.

Ivanalee, A. S., Yudaniayanti, I. S., Yunita, M. N., Triakoso, N., Hamid, I. S., & Saputro, A. L. (2018). Efektivitas sugar dressing (100% gula) dalam meningkatkan kepadatan kolagen pada proses penyembuhan luka bakar buatan pada kulit tikus putih (Rattus norvegicus) jantan. Jurnal Medik Veteriner, 1(3), 134.

Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Burn injury. Nat Rev Dis Primers. 2020 Feb 13;6(1):11. doi: 10.1038/s41572-020-0145-5. PMID: 32054846; PMCID: PMC7224101.

Kara, Y. A. (2018). Burn Etiology and Pathogenesis. InTech.doi:10.5772/intechopen.71379

Kawi, J. S., Yulianti, E., Limanan, D., & Ferdinal, F. (2021, December). Phytochemicals profiling and total antioxidant capacity of cinnamon bark extract (Cinnamomum burmanii). In 1st Tarumanagara International Conference on Medicine and Health (TICMIH 2021) (pp. 33-38). Atlantis Press.

Krutmann, J., Morita, A., & Chung, J. H. (2012). Sun exposure: what molecular photodermatology tells us about its good and bad sides. Journal of Investigative Dermatology, 132(3), 976-984.

Landén NX, Li D, Ståhle M. Transition from inflammation to proliferation: a critical step during wound healing. Cell Mol Life Sci. 2016 Oct;73(20):3861-85. doi: 10.1007/s00018-016-2268-0. Epub 2016 May 14. PMID: 27180275; PMCID: PMC5021733.

Layuck, A. R., Lintong, P. M., & Loho, L. L. (2015). Pengaruh pemberian air perasan jeruk nipis (Citrus aurantifolia) terhadap jumlah pigmen melanin kulit mencit (Mus musculus) yang dipaparkan sinar matahari. eBiomedik, 3(1).

Lephart, E. D. (2018). Equol’s anti-aging effects protect against environmental assaults by increasing skin antioxidant defense and ECM proteins while decreasing oxidative stress and inflammation. Cosmetics, 5(1), 16.

Lopez-Ojeda W, Pandey A, Alhajj M, et al. Anatomy, Skin (Integument) [Updated 2022 Oct 17]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK441980/

Mathes SH, Ruffner H, Graf-Hausner U. The use of skin models in drug development. Adv Drug Deliv Rev. 2014;69–70:81–102. https://doi.org/10.1016/j.addr.2013.12.006.

Mathew-Steiner SS, Roy S, Sen CK. Collagen in Wound Healing. Bioengineering (Basel). 2021 May 11;8(5):63. doi: 10.3390/bioengineering8050063. PMID: 34064689; PMCID: PMC8151502.

Mesa-Arango, A. C., Flórez-Muñoz, S. V., & Sanclemente, G. (2017). Mechanisms of skin aging. Iatreia, 30(2), 160-170.

McBride, W. H., & Schaue, D. (2020). Radiation‐induced tissue damage and response. The Journal of pathology, 250(5), 647-655.

Mohania, D., Chandel, S., Kumar, P., Verma, V., Digvijay, K., Tripathi, D., ... & Shah, D. (2017). Ultraviolet radiations: Skin defense-damage mechanism. Ultraviolet Light in Human Health, Diseases and Environment, 71-87.

Mehta K, Gyedu A, Otupiri E, Donkor P, Mock C, Stewart B. Incidence of childhood burn injuries and modifiable household risk factors in rural Ghana: A cluster-randomized, population-based, household survey. Burns. 2021 Jun;47(4):944-951. doi: 10.1016/j.burns.2020.09.001. Epub 2020 Oct 5. PMID: 33077331; PMCID: PMC8019680.

Nizamoglu, M., Tan, A., Vickers, T., Segaren, N., Barnes, D., & Dziewulski, P. (2016). Cold burn injuries in the UK: the 11-year experience of a tertiary burns centre. Burns & Trauma, 4.

O'Connor, C., Courtney, C., & Murphy, M. (2021). Shedding light on the myths of ultraviolet radiation in the COVID‐19 pandemic. Clinical and Experimental Dermatology, 46(1), 187-188.

Pozzi A, Yurchenco PD, Iozzo RV. The nature and biology of basement membranes. Matrix Biol. 2017;57–58:1–11. https://doi.org/10.1016/j.matbio.2016.12.009.

Ramponi, D. R. (2017). Chemical burns of the eye. Advanced emergency nursing journal, 39(3), 193-198.

Rahayu, D. U. C., Hakim, R. A., Mawarni, S. A., & Satriani, A. R. (2022). Indonesian Cinnamon (Cinnamomum burmannii): Extraction, Flavonoid Content, Antioxidant Activity, and Stability in the Presence of Ascorbic Acid. Cosmetics, 9(3), 57. https://doi.org/10.3390/cosmetics9030057

Rao PV, Gan SH. Cinnamon: a multifaceted medicinal plant. Evid Based Complement Alternat Med. 2014;2014:642942. doi: 10.1155/2014/642942. Epub 2014 Apr 10. PMID: 24817901; PMCID: PMC4003790.

Reilly, D. M., & Lozano, J. (2021). Skin collagen through the lifestages: Importance for skin health and beauty. undefined, 8, N-A.

Rittié, L., & Fisher, G. J. (2015). Natural and sun-induced aging of human skin. Cold spring harbor perspectives in medicine, 5(1), a015370.

Roger, M., Fullard, N., Costello, L., Bradbury, S., Markiewicz, E., O’Reilly, S., … Przyborski, S. (2019). Bioengineering the microanatomy of human skin. Journal of Anatomy. doi:10.1111/joa.12942

Savoji, H., Godau, B., Hassani, M. S., & Akbari, M. (2018). Skin tissue substitutes and biomaterial risk assessment and testing. Frontiers in bioengineering and biotechnology, 6, 86.

Sirait, T. S., Arianto, A., & Dalimunthe, A. (2023). Phytochemical Screening of Cinnamon Bark (Cinnamomum burmanii)(C. Ness & T. Ness) C. Ness ex Blume Ethanol Extract and Antioxidant Activity Test with DPPH (2, 2-diphenyl-1-picrylhydrazyl) Method. International Journal of Science, Technology & Management, 4(1), 254-259.

Sklar, L. R., Almutawa, F., Lim, H. W., & Hamzavi, I. (2012). Effects of ultraviolet radiation, visible light, and infrared radiation on erythema and pigmentation: a review. Photochemical & Photobiological Sciences, 12, 54-64.

Strong AL, Neumeister MW, Levi B. Stem cells and tissue engineering: regeneration of the skin and its contents. Clin Plast Surg. 2017;44:635–50. https://doi.org/10.1016/j.cps.2017.02.020.

Tavares Pereira, D. D. S., Lima-Ribeiro, M. H. M., de Pontes-Filho, N. T., Carneiro-Leão, A. M. D. A., & Correia, M. T. D. S. (2012). Development of animal model for studying deep second‐degree thermal burns. BioMed Research International, 2012(1), 460841.

Wang, P. -W., Hung, Y. -C., Lin, T. -Y., Fang, J. -Y., Yang, P. -M., Chen, M. -H., & Pan, T. -L. (2019). Comparison of the Biological Impact of UVA and UVB upon the Skin with Functional Proteomics and Immunohistochemistry. Antioxidants, 8(12), 569. https://doi.org/10.3390/antiox8120569

Watson, M., Holman, D. M., & Maguire-Eisen, M. (2016, August). Ultraviolet radiation exposure and its impact on skin cancer risk. In Seminars in oncology nursing (Vol. 32, No. 3, pp. 241-254). WB Saunders

Wolf, S. T., Berry, C. W., Stanhewicz, A. E., Kenney, L. E., Ferguson, S. B., & Kenney, W. L. (2019). Sunscreen or simulated sweat minimizes the impact of acute ultraviolet radiation on cutaneous microvascular function in healthy humans. Experimental physiology, 104(7), 1136-1146.

Xue, M., & Jackson, C. J. (2015). Extracellular matrix reorganization during wound healing and its impact on abnormal scarring. Advances in wound care, 4(3), 119-136.

Young AR, Claveau J, Rossi AB. Ultraviolet radiation and the skin: photobiology and sunscreen photoprotection. J Am Acad Dermatol. 2017;76(3):S100–9. doi:10.1016/j.jaad.2016.09.038

Żwierełło W, Piorun K, Skórka-Majewicz M, Maruszewska A, Antoniewski J, Gutowska I. Burns: Classification, Pathophysiology, and Treatment: A Review. Int J Mol Sci. 2023 Feb 13;24(4):3749. doi: 10.3390/ijms24043749. PMID: 36835171; PMCID: PMC9959609.