UJI EFEKTIVITAS ANTIHIPERGLIKEMIK TEPUNG BATANG KROKOT (PORTULACA OLERACEA L.) PADA TIKUS MODEL DIABETES MELLITUS TIPE 2
DOI:
https://doi.org/10.31004/prepotif.v8i3.38337Keywords:
berat badan, diabetes melitus tipe 2, glukosa darah puasa, tepung batang krokotAbstract
Diabetes melitus tipe 2 meningkatkan produksi radikal bebas yang menyebabkan peningkatan kadar glukosa darah (GDP) dan penurunan berat badan (BB . Tepung batang krokot berpotensi menjadi bahan pangan yang mempunyai efek antihiperglikemik karena mengandung antosioksidan serta tinggi serat. Penelitian ini bertujuan untuk mengetahui efektivitas antihiperglikemik tepung batang krokot melalui pemeriksaan kadar glukosa darah puasa dan berat badan pada tikus wistar DMT2. Jenis penelitian ini adalah eksperimen. Sebanyak tiga puluh tikus wistar jantan model DMT2 dibagi menjadi 6 kelompok : Tikus sehat dengan pakan standar (K), Tikus DMT2 dengan pemberian pakan standar (P1), pemberian pakan standar dan metformin dosis 9 mg/200gBB (P2), pemberian pakan standar dan tepung batang krokot dosis 176mg/200gBB (P3), 352mg/200gBB (P4) dan dosis 528mg/200gBB (P5). Pengukuran kadar GDP dan BB dilakukan pada hari ke-7 intervensi. Data dianalisis menggunakan uji t-test dan One way Anova dengan p < 0,05. Rerata penurunan kadar GDP pada hari ke-7 kelompok K (0,77 ± 0,29), P1 (0,68 ± 0,46 mg/dl), P2 (-116,08 ± 0,53 mg/dl) (p=0,009), P3 (-114,55 ± 9,03 mg/dl) , P4 (-127,17 ± 16,01 mg/dl, P5 (-122,11 ± 2,58 mg/dl). Pemberian tepung batang krokot dengan dosis 176, 352 dan 528mg/200gBB secara signifikan menurunkan kadar GDP, dan meningkatkan BB tikus wistar DMT2 mulai di hari ke-7. Pemberian tepung batang krokot 352mg/200gBB paling efektif menurunan kadar GDP dan meningkatkan BB pada tikus wistar DMT2References
American Diabetes Associated. (2015). Nutrition Therapy Recommendations for the Management of Adults With Diabetes.Diabetes Care 37(Supplement_1): S120–43.
Eleazu CO, Eleazu KC, Chukwuma S and Essien UN. (2013). Review of The Mechanism of Cell Death Resulting from Streptozotocin Challenge in Experimental Animals , Its Practical Use and Potential Risk to Humans. Journal of Diabetes & Metabolic Disorders 12:60.
Ghasemi A, Khalifi S and Jedi S. (2014). Streptozotocin-Nicotinamide-Induced Rat Model of Type 2 Diabetes (Review). Acta Physiologica Hungarica 101(4): 408–20.
Grace MH, Ribnicky DM, Kuhn P, Poulev A, Logendra S, Yousef GG, Raskin I and Lila MA. (2009). Hypoglycemic Activity of A Novel Anthocyanin-Rich Formulation from Lowbush Blueberry, Vaccinium Angustifolium Aiton. Phytomedicine 16(5):406–415.
Graham ML, Janecek JL, Kittredge JA, Hering BJ and Schuurman HJ.(2011). The Streptozotocin-Induced Diabetic Nude Mouse Model?: Differences between Animals from Different Sources. Comparative Medicine 61(4):56–360
Guo X, Wang Y, Wang K, Ji B and Zhou F. (2018). Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 19(7):559–569.
Nagarchi K, Ahmde S, Sabus A and Saheb SH. (2015). Effect of Streptozotocin on Glucose levels in Albino Wister Rats. J. Pharm. Sci 7(2):67–69.
Palupi FD. (2018). Pengaruh Dosis dan Lama Pemberian Ekstrak Etanol Pegagan (Centella asiatica) terhadap Derajat Insulin pada Pankreas Tikus Model Diabetes Mellitus Tipe 2. Tesis, Universitas Sebelas Maret, Surakarta
Pandya KG, Patel MR. and Lau-Cam CA. (2010). Comparative study of the binding characteristics to and inhibitory potencies towards PARP and in vivo antidiabetogenic potencies of taurine, 3-aminobenzamide and nicotinamide. J. Biomed. S17 (1).
Takikawa M, Inoue S, Horio F, and Tsuda T. (2010). Dietary Anthocyanin-Rich Bilberry Extract Ameliorates Hyperglycemia and Insulin Sensitivity via Activation of AMP-Activated Protein Kinase in Diabetic Mice". The Journal of Nutrition 1(3) : 527–33.
Zafar M and Naeem-Ul-Hassan S. (2010). Effects of STZ-Induced Diabetes on the Relative Weights of Kidney , Liver and Pancreas in Albino Rats?: A Comparative Study.Int. J. Morphol 28(1):135–142.
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