Antimicrobial Test of Dayak Onions (Eleutherine Palmifolia (L.) Merr) Leaves on The Growth Of Streptococcus Mutans

Abstract

Streptococcus mutans bacteria is a gram-positive bacterium that can cause infective endocarditis. In USA, the incidence of endocarditis ranges from 3 to 10 cases per 100,000 population per year. Infections caused by bacteria can be treated with antibiotics. Dayak onion leaves have been used as medicinal ingredients because they contain phytochemicals such as flavonoids, saponins, alkaloids, tannins and triterpenoids which act as antibacterial. This study aims to identify the antibacterial properties of Dayak onion leaaves extract against Streptococcus mutans bacteria in vitro. This study used a true experimental design with the treatment of Dayak onion leaves  against Streptococcus mutans bacteria to test its antibacterial activity using the well diffusion method with concentrations of 25%, 50%, and 75%. The average measurement results with concentrations of 75%, 50%, and 25% were 25,41 mm, 23,20 mm, and 22,32 mm respectively. The positive control used in the experiment was the Amoxicillin antibiotic with an average inhibition of 18,02 mm and the negative control was Ampicillin antibiotic with an average inhibition of 10,14 mm. Meanwhile, the placebo control was 10% DMSO without an inhibition zone for bacteria. Based on the study results, the extract of Dayak onion leaves with a concentration of 25%, 50%, and 75% have antibacterial activity against Streptococcus mutans bacteria.