EKSTRAK PENTA HERBS FORTE : KAPASITAS ANTIOKSIDAN, UJI TOKSISITAS DAN PENGARUH TERHADAP KADAR GSH HATI TIKUS YANG DIINDUKSI HIPOKSIA SISTEMIK KRONIK
DOI:
https://doi.org/10.31004/jkt.v6i4.54183Keywords:
antioksidan, ABTS, glutation, hipoksia sistemik, penta herbs forteAbstract
Hipoksia sistemik kronik dapat meningkatkan pembentukan senyawa oksigen reaktif (ROS) yang berperan dalam terjadinya stres oksidatif dan kerusakan jaringan, khususnya pada organ hati. Kondisi ini menyebabkan penurunan aktivitas sistem antioksidan endogen, salah satunya glutation tereduksi (GSH). Penta Herbs Forte merupakan kombinasi ekstrak lima tanaman herbal, yaitu jahe merah, temulawak, meniran, sambiloto, dan sembung, yang diketahui memiliki potensi antioksidan. Penelitian ini bertujuan untuk mengevaluasi kapasitas antioksidan ekstrak Penta Herbs Forte menggunakan metode ABTS, uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT), serta pengaruhnya terhadap kadar GSH hati tikus yang diinduksi hipoksia sistemik kronik. Penelitian ini merupakan studi eksperimental yang terdiri dari uji in vitro dan in vivo. Uji kapasitas antioksidan dilakukan dengan metode ABTS dan dinyatakan sebagai nilai IC₅₀. Uji toksisitas dilakukan menggunakan BSLT untuk menentukan nilai LC₅₀. Uji in vivo dilakukan pada tikus Sprague Dawley jantan yang dibagi ke dalam kelompok kontrol dan kelompok uji yang diberikan ekstrak Penta Herbs Forte, kemudian diinduksi hipoksia sistemik kronik. Kadar GSH hati diukur menggunakan metode Ellman. Hasil penelitian menunjukkan bahwa ekstrak Penta Herbs Forte memiliki kapasitas antioksidan yang tinggi berdasarkan uji ABTS dan menunjukkan aktivitas toksik pada uji BSLT. Kadar GSH hati mengalami penurunan seiring lamanya paparan hipoksia pada kedua kelompok. Namun, kadar GSH hati pada kelompok yang diberikan ekstrak Penta Herbs Forte lebih tinggi dibandingkan kelompok kontrol. Dapat disimpulkan bahwa ekstrak Penta Herbs Forte memiliki aktivitas antioksidan dan berperan dalam mempertahankan kadar GSH hati pada kondisi hipoksia sistemik kronik.References
Andrés, C. M. C., Pérez de la Lastra, J. M., Juan, C. A., Plou, F. J., & Pérez-Lebeña, E. (2024). Antioxidant Metabolism Pathways in Vitamins, Polyphenols, and Selenium: Parallels and Divergences. International Journal of Molecular Sciences, 25(5), 2600. https://doi.org/10.3390/ijms25052600
Azizah, S. (2023). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Jahe Merah (Zingiber Officinale Var. Rubrum) Dengan Metode Abts. Jurnal Borneo Cendekia; Vol 7 No 1, (2023): Jurnal Borneo Cendekia. https://www.journal.stikesborneocendekiamedika.ac.id/index.php/jbc/article/view/379
Banerjee, P., Gaddam, N., Chandler, V., & Chakraborty, S. (2023). Oxidative Stress–Induced Liver Damage and Remodeling of the Liver Vasculature. The American Journal of Pathology, 193(10), 1400–1414. https://doi.org/10.1016/j.ajpath.2023.06.002
Bekkouch, O., Bekkouch, A., Elbouny, H., Touiss, I., Assri, S. El, Choukri, M., Bourhia, M., Kim, K., Kang, S., Choi, M., Choi, J., Kim, H. J., Ahn, C.-H., Park, M. N., Kim, B., & Amrani, S. (2025). Combinatorial effects of Zingiber officinale and Citrus limon juices: Hypolipidemic and antioxidant insights from in vivo, in vitro, and in silico investigations. PLOS One, 20(9), e0319721. https://doi.org/10.1371/journal.pone.0319721
Chang, X., Zhou, S., Yan, Z., Zhang, Q., Liu, J., Wang, Y., Guan, X., Wu, Q., & Liu, R. (2025). Potential Candidates of Natural Antioxidants From Herbs for Treating Lung Disorders: Focus on Redox Balance and Natural Products. Phytotherapy Research, 39(8), 3353–3385. https://doi.org/10.1002/ptr.70008
Chen, M., Limanan, D., Yulian, E., & Ferdinal, F. (2022). Pengaruh daun ara terhadap kadar glutation jantung tikus yang diinduksi hipoksia sistemik kronik. Jurnal Media Sains dan Teknologi Kesehatan, 6(1), 33–40.
Chu, Q., Gu, X., Zheng, Q., & Zhu, H. (2022). Regulatory mechanism of HIF-1α and its role in liver diseases. Annals of Translational Medicine.
Farías, J. G., Herrera, E. A., Carrasco-Pozo, C., Sotomayor-Zárate, R., Cruz, G., Morales, P., & Castillo, R. L. (2016). Pharmacological models and approaches for pathophysiological conditions associated with hypoxia and oxidative stress. Pharmacology & Therapeutics, 158, 1–23. https://doi.org/10.1016/j.pharmthera.2015.11.006
Juan, C. A., Pérez de la Lastra, J. M., Plou, F. J., & Pérez-Lebeña, E. (2021). The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences, 22(9), 4642. https://doi.org/10.3390/ijms22094642
Liang, Y., Ruan, W., Jiang, Y., Smalling, R., Yuan, X., & Eltzschig, H. K. (2023). Interplay of hypoxia-inducible factors and oxygen therapy in cardiovascular medicine. Nature Reviews Cardiology, 20(11), 723–737. https://doi.org/10.1038/s41569-023-00886-y
Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity. International Journal of Molecular Sciences, 22(7), 3380.
Omar, J. M., Hai, Y., & Jin, S. (2022). Hypoxia-induced factor and its role in liver fibrosis. PeerJ, 10, e14299. https://doi.org/10.7717/peerj.14299
Perdana, P. R. (2022). Aktivitas imunomodulator ekstrak herba meniran. Jurnal Farmagazine, 9(1), 50–54.
Pérez-Torres, I., Castrejón-Téllez, V., Soto, M. E., Rubio-Ruiz, M. E., Manzano-Pech, L., & Guarner-Lans, V. (2021). Oxidative Stress, Plant Natural Antioxidants, and Obesity. International Journal of Molecular Sciences, 22(4), 1786. https://doi.org/10.3390/ijms22041786
Pranata, G., Indriaty, I., & S. Pandia, E. (2025). Uji Kadar Total Tanin dari Ekstrak Daun Sembung (Blumea balsamifera) serta Toksisitasnya Dengan mentode BSLT. Jurnal sosial dan sains, 5(6), 1703–1712. https://doi.org/10.59188/jurnalsosains.v5i6.32260
Rahmat, E., Lee, J., & Kang, Y. (2021). Javanese Turmeric (Curcuma xanthorrhiza Roxb.): Ethnobotany, Phytochemistry, Biotechnology, and Pharmacological Activities. Evidence-Based Complementary and Alternative Medicine, 2021, 1–15. https://doi.org/10.1155/2021/9960813
Rahmi, A., Afriani, T., Hevira, L., & Widiawa, W. (2021). Uji aktivitas antioksidan dan fenolik total ekstrak daun sembung. Jurnal Riset Kimia, 12(2).
Ramdan, S. R. K., Asih, A. C., Yusuf, A. L., Nugraha, D., Indriastuti, M., & Wahlanto, P. (2024). Isolasi dan Identifikasi Kurkumin Ekstrak Etanol Temulawak (Curcuma xanthorriza Roxb.) dengan Metode Kromatografi Kolom. Pharmacy Genius, 3(3), 172–179. https://doi.org/10.56359/pharmgen.v3i3.427
Rusmana, D., Wahyudianingsih, R., Elisabeth, M., Balqis, B., Maesaroh, M., & Widowati, W. (2017). Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin. The Indonesian Biomedical Journal, 9(2), 84. https://doi.org/10.18585/inabj.v9i2.281
Widhiantara, I. G., & Jawi, I. M. (2021). Phytochemical composition and health properties of Blumea balsamifera. Veterinary World, 14(5), 1185–1194.
Zammel, N., Saeed, M., Bouali, N., Elkahoui, S., Alam, J. M., Rebai, T., Kausar, M. A., Adnan, M., Siddiqui, A. J., & Badraoui, R. (2021). Antioxidant and Anti-Inflammatory Effects of Zingiber officinale roscoe and Allium subhirsutum: In Silico, Biochemical and Histological Study. Foods, 10(6), 1383. https://doi.org/10.3390/foods10061383
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