POOLED SERA SEBAGAI ALTERNATIF BAHAN KONTROL PADA PEMERIKSAAN LABORATORIUM KLINIK : TINJAUAN LITERATUR NARATIF
DOI:
https://doi.org/10.31004/jkt.v6i2.46431Keywords:
Pooled sera, Qualiti Control, Bahan kontrol, Kontrol KomersialAbstract
Setiap laboratorium klinik harus melakukan Quality Control pada setiap parameter pemeriksaan agar hasil pemeriksaan yang didapatkan adalah hasil yang akurat, presisi, dan bermutu tinggi dan terjamin. Quality Control dapat dilakukan setiap hari. Akan tetapi, harga bahan kontrol yang relatif mahal dan tingkat kesulitan untuk mendapatkan bahan kontrol yang cukup tinggi menjadi masalah yang harus dihadapi oleh laboratorium. Pooled sera hadir sebagai alternatif bahan kontrol yang dinilai lebih ekonomis, mudah dibuat, dan tidak berbahaya sehingga cocok digunakan sebagai bahan kontrol teruatama oleh laboratorium yang memiliki akses dan sumber daya yang terbatas. Pooled sera dilaporkan dapat menjadi alternatif bahan kontrol pengganti bahan kontrol komersial pada pemeriksaan Glukosa Darah, Trigliserida, Kolesterol, HDL, CRP, SGPT, Albumin, dan Protein Total. Pooled sera memiliki potensi yang besar sebagai alternatif bahan kontrol yang dapat menggantikan kontrol komersial terutama pada pemeriksaan glukosa darah.References
Anggra, Wiwit Nia, Ibrahim, and Rahmi Novita Yusuf. 2021. “Comparasion Between Pooled sera Material and Commercial Serum on the Accuracy of Triglyceride Check.” Advances in Health Sciences Research 39(SeSICNiMPH):330–34.
Aziza, Heppy Nur, and Aji Bagus Widyantara. 2022. “Profil Kadar Serum Glutamic Oxaloacetic Transaminase (Sgot) Dan Serum Glutamic Pyruvic Transaminase (Sgpt) Pada Pasien Demam Berdarah Dengue.” 6–13.
Bastian, Bastian, Maria Ulva, and Inoy Rizky Rahmadani. 2024. “Pemanfaatan Pooled sera Sebagai Bahan Kontrol Pemantapan Mutu Internal Pemeriksaan SGPT.” Jurnal Kesehatan Vokasional 9(2):115–25. doi: 10.22146/jkesvo.95611.
BIO-RAD Laboratories. 2023. Laboratory Quality Control Materials QC Workbook Series. Bio-Rad Laboratories, Inc.
Devi, Anita, and Anjana Negi. 2023. “Pooled sera as an Alternative to Commercial Internal Quality Control in Clinical Laboratories.” Journal of Clinical and Diagnostic Research 1–4. doi: 10.7860/jcdr/2023/64088.18567.
Diona, Adara Aurelia, and Ally Kafesa. 2024. “Analisis Stabilitas Glukosa, Trigliserida Dan Albumin Pada Pooled Serum.” MAHESA: MALAHAYATI HEALTH STUDENT JOURNAL 4:5128–37.
Fatmariza, Aulia Risqi, Angga Eka Kurniawan, and Nofita Sari. 2024. “Stabilitas Pooled sera Etilen Glikol Dan Pooled Plasma NaF Sebagai Bahan Kontrol Pemeriksaan Glukosa Stability of Pooled sera Ethylene Glycol and Pooled Plasma NaF as Control Materials for Glucose Testing.” Jurnal Riset Pengembangan Dan Pelayanan Kesehatan 3(2).
Fauziah, Suci, Ani Riyani, Sonny Feisal Rinaldi, and Nani Kurnaeni. 2019. “Pada Pooled sera Yang Ditambah Etilen Glikol.” Jurnal Riset Kesehatan Poltekkes Depkes Bandung 11(2):287–93.
Food and Drug Administration. 2007. Guidance for Industry and FDA Staff Assayed and Unassayed Quality Control Material. U.S. Department of Health and Human Services Food and Drug Administration Center for Devices and Radiological Health Office of In Vitro Diagnostic Device Evaluation and Safety.
Kementerian Kesehatan RI. 2010. “Peraturan Menteri Kesehatan Republik Indonesia No 411/MENKES/PER/III/2010 Tentang Laboratorium Klinik.” Pusat Komunikasi Publik Departemen Kesehatan 210.
Kementerian Kesehatan RI. 2013. Peraturan Menteri Kesehatan Republik Indonesia Nomor 13 Tahun 2013 Tentang Pedoman Manajemen Terpadu Pengendalian Tuberkuloasis Resistan Obat. Kementerian Kesehatan Republik Indonesia.
Kulkarni, Sweta, Shema Alain Pierre, and Ramachandran Kaliaperumal. 2020. “Efficacy of Pooled Serum Internal Quality Control in Comparison with Commercial Internal Quality Control in Clinical Biochemistry Laboratory.” Journal of Laboratory Physicians 12(03):191–95. doi: 10.1055/s-0040-1721151.
Lhajjam, H., H. Azzouzi, N. Sefiani, H. Elmrabet, and N. Ihendyane. 2023. “The Importance of the Internal Quality Controls in Medical Laboratories to Ensure High Quality Results.” Jornal Brasileiro de Patologia e Medicina Laboratorial 59(2):7–17. doi: 10.1900/JBPML.2023.59.02.002.
Made, Ida Ayu, Aris Wibudi, roy panusunan Sibarani, made ratna Saraswati, I. made Pande Dwipayana, Harli Amir Mahmudji, Dicky L. Tahapary, M. Aron Pase, Yensuari, and Tania Tedjo Minuljo. 2021. Pedoman Pemantauan Glukosa Darah Mandiri. PB Perkeni.
Maretha, Indria Putri. 2023. “Perbandingan Hasil Pemeriksaan Kadar Albumin Menggumnakan Plasma EDTA Berdasarkan Lama Waktu Inkubasi Tahun 2023.” Politeknik Kesehatan Palembang.
Maulidiyanti, Ellies Tunjung Sari, Nur Vita Purwaningsih, Rahma Widiyastuti, Rinza Rahmawati Samsudin, and Anindita Riesti Retno Arimurti. 2021. “The Effect of Storage Time for Pooled sera on Freezers on the Quality of Clinical Chemical Examination.” Medicra (Journal of Medical Laboratory Science/Technology) 4(2):78–82. doi: 10.21070/medicra.v4i2.1613.
Park, Byoungjin, and Yong Jae Lee. 2025. “Extremely High HDL Cholesterol Paradoxically Increases the Risk of All-Cause Mortality in Non-Diabetic Males from the Korean Population : Korean Genome and Epidemiology Study-Health Examinees ( KoGES-HEXA ) Cohorts.” (May):1–11. doi: 10.3389/fmed.2025.1534524.
Pirzado, Ali, Jhahzad Jiskani, Muhammad Sallen, and Naz Lubna. 2021. “Determination of Pooled Serum as a Better and Cheap Method for Internal Quality Control in Clinical Laboratory.” Linguística Antverpiensia (3):2561–74.
Putri, Deli Rahayu. 2020. “Uji Stabilitas Pooled sera Dengan Dan Tanpa Penambahan Etilen Glikol Terhadap Pemeriksaan Kolesterol.” Sekolah Tinggi Ilmu Kesehatan Perintis Padang.
Quam, Elsa F. 2025. “BASIC QC PRACTICES.” Retrieved June 2, 2025 (https://westgard.com/lessons/basic-qc-practices-l/lesson13.html).
Ramdhani, Fitria Hariati, Dwi Purbayanti, and Putri Astuti. 2023. “Uji Stabilitas Pooled sera Yang Disimpan Di Suhu 2 - 8oc Pada Pemeriksaan Albumin The Pooled sera Stability Test Is Stored at a Temperature of 2 - 8oc on Albumin.” Borneo Journal Of Medical Laboratory Technology 6(1):371–75.
Ren, Xiaoli, Mengying Chen, Liyou Lian, Huimin Xia, Wei Chen, Shengjie Ge, Lijuan Yang, Qingxi Jiang, Xuejiang Gu, Bo Yang, and Xiang Hu. 2025. “The Triglyceride-Glucose Index Is Associated with a Higher Risk of Hypertension: Evidence from a Cross-Sectional Study of Chinese Adults and Meta-Analysis of Epidemiology Studies.” Frontiers in Endocrinology 16(February). doi: 10.3389/fendo.2025.1516328.
Sari, Rindra Sekar, and Titin Aryani. 2021. “Gambaran Hasil Kontrol Kualitas Pemeriksaan Glukosa Dan Kolesterol Pada Pooled sera Berdasarkan Variasi Penyimpanan: Literature Review.” Universitas ’Aisyiyah Yogyakarta.
Sari, Yulia Ratna, Fanny Jesica, and Niken Niken. 2023. “Comparison between Pooled sera and Coomercial Serum on the Accuracy of Triglyceride Assessment.” International Journal of Multidisciplinary Approach Research and Science 1(03):375–80. doi: 10.59653/ijmars.v1i03.220.
Doni Setiawan, Ranita Nurjamilah, Nabil Ridla, and Firdaus Ary. 2024. “STABILITAS POOLED SERA SEBAGAI ALTERNATIF BAHAN KONTROL POSITIF PEMERIKSAAN C-REACTIVE PROTEIN.” Program Studi D3 Teknologi Laboratorium Medis, STIKes Muhammadiyah Ciamis, Jawa Barat, Indonesia.
Siregar, Maria Tuntun, Wieke Sri Wulan, Doni Setiawan, and Anik Nuryanti. 2018. Bahan Ajar Teknologi Laboratorium Medik (TLM) Kendali Mutu. 1st ed. Pusat Pendidiakn Sumber Daya Manusia Kesehatan Badan Pengembangan Dan Pemberdayaan Sumber Daya Manusia Kesehatan Kementerian Kesehatan Republik Indonesia.
Tewabe, Haymanot, Asaye Mitiku, and Abebe Yenesew. 2024. “Validation of the Efficacy of Pooled Serum for Serum Glucose Inhouse Quality Control Material in Comparison with Commercial Internal Quality Control in Clinical Chemistry Laboratory.” Practical Laboratory Medicine 39(March):e00377. doi: 10.1016/j.plabm.2024.e00377.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Ahmat Rediansya Putra
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
