AKTIVITAS ANTIFUNGI EKSTRAK ETANOL NEPHTHEA SP. TERHADAP MALASSEZIA FURFUR DAN TRICHOPHYTON RUBRUM
DOI:
https://doi.org/10.31004/jkt.v6i2.45105Keywords:
antifungal, Malassezia, Nephthea, soft coral, TrichophytonAbstract
Infeksi mikosis termasuk salah satu infeksi yang sering terjadi di Indonesia. Infeksi mikosis menjadi sulit diobati akibat terjadinya resistensi fungi terhadap antifungi sintetik. Untuk itu, diperlukan pencarian obat berasal dari alam sebagai alternatif dalam pengobatan infeksi mikosis. Nephthea sp. merupakan jenis karang lunak yang berasal dari famili Nephtheidae yang tersebar luas di perairan laut Indo-Pasifik. Nephthea sp. mengandung senyawa metabolit sekunder yang berpotensi sebagai agen antimikroba. Penelitian ini bertujuan untuk menganalisis aktivitas antifungi dari ekstrak etanol 96% Nephthea sp. terhadap Malassezia furfur dan Trichophyton rubrum. Ekstrak etanol Nephthea sp. diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Ekstrak etanol Nephthea sp. di uji secara kualitatif kandungan senyawa metabolit sekundernya, meliputi alkaloid, tanin, flavonoid, saponin, dan triterpenoid. Ekstrak Nephthea sp. juga diuji kemampuannya menghambat pertumbuhan fungi Malassezia furfur dan Trichophyton rubrum menggunakan metode difusi cakram. Aktivitas antifungi ditunjukkan dengan zona bening (zona inhibisi) yang terbentuk di sekitar kertas cakram. Ketokonazol (15 µg) digunakan sebagai kontrol positif dan DMSO 10% sebagai kontrol negatif. Data skrining senyawa metabolit sekunder dan aktivitas antifungi ekstrak etanol Nephthea sp. dianalisis secara deskriptif. Berdasarkan uji skrining senyawa metabolit sekunder menunjukkan bahwa ekstrak etanol Nephthea sp. positif mengandung alkaloid, saponin, dan triterpenoid. Ekstrak etanol Nephthea sp. memiliki aktivitas antifungi terhadap Trichophyton rubrum pada konsentrasi 1000 mg/mL, 750 mg/mL, dan 500 mg/mL. Sedangkan aktivitas antifungi terhadap Malassezia furfur tidak terdeteksi. Dengan demikian, dapat disimpulkan bahwa ekstrak etanol Nephthea sp. mengandung senyawa metabolit sekunder yang berpotensi sebagai antifungi terhadap Trichophyton rubrum dengan daya hambat bersifat moderat.References
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