REVIEW ARTIKEL : PRODUKSI VAKSIN COVID-19 DENGAN METODE REKAYASA GENETIK
DOI:
https://doi.org/10.31004/jkt.v6i2.45103Keywords:
Bioteknologi, COVID-19, Metode, Rekayasa Genetik, VaksinAbstract
Review artikel ini meninjau perkembangan teknologi bioteknologi dalam produksi vaksin COVID-19 menggunakan metode rekayasa genetik. Tujuan review artikel ini yaitu untuk mengidentifikasi inovasi baru seperti vaksin berbasis DNA dan mRNA, serta sistem diagnostik CRISPR-Cas, yang menunjukkan potensi signifikan dalam menangani pandemic COVID-19. Metode yang digunakan adalah narrative review dengan pemilihan artikel dari database seperti Google Scholar dan PubMed. Hasil ulasan menunjukkan bahwa vaksin RNA dan DNA mampu memicu respons imun spesifik dan kuat, dengan keunggulan dalam menghadapi varian baru SARS-CoV-2. Di sisi lain, teknologi CRISPR-Cas berperan ganda sebagai alat diagnostik cepat dan strategi antivirus yang efektif. Diharapkan bioteknologi dapat mengembangankan vaksin dan sistem diagnostik, memberikan dampak penting bagi upaya global dalam mengatasi pandemi COVID-19.References
Abbott, T. R., Dhamdhere, G., Liu, Y., Lin, X., Goudy, L., Zeng, L., ... & Qi, L. S. (2020). Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza. Cell, 181(4), 865-876.
Alamri, S. S., Alluhaybi, K. A., Alhabbab, R. Y., Basabrain, M., Algaissi, A., Almahboub, S., ... & Hashem, A. M. (2021). Synthetic SARS-CoV-2 spike-based DNA vaccine elicits robust and long-lasting th1 humoral and cellular immunity in mice. Frontiers in microbiology, 12, 727455.
Borah, P., Deb, P. K., Al-Shar’i, N. A., Dahabiyeh, L. A., Venugopala, K. N., Singh, V., & Jaradat, D. S. M. (2021). Perspectives on RNA vaccine candidates for COVID-19. Frontiers in Molecular Biosciences, 8, 635245.
Cooper, A., Sidaway, A., Chandrashekar, A., Latta, E., Chakraborty, K., Yu, J., ... & Barouch, D. H. (2022). A genetically engineered, stem-cell-derived cellular vaccine. Cell Reports Medicine, 3(12).
de Queiroz, N. M. G., Marinho, F. V., Chagas, M. A., Leite, L. C., Homan, E. J., de Magalhaes, M. T., & Oliveira, S. C. (2020). Vaccines for COVID-19: perspectives from nucleic acid vaccines to BCG as delivery vector system. Microbes and Infection, 22(10), 515-524.
Guo, YR, Cao, QD, Hong, ZS, Tan, YY, Chen, SD, Jin, HJ, dkk. (2020). Asal, penularan, dan terapi klinis pada wabah penyakit virus korona 2019 (COVID-19)— pembaruan status. Mil. Med. Res. 7, 11–10. doi:10.1186/s40779-020-00240-0.
Hogan, M. J., & Pardi, N. (2022). mRNA vaccines in the COVID-19 pandemic and beyond. Annual review of medicine, 73(1), 17-39.
Hou, T., Zeng, W., Yang, M., Chen, W., Ren, L., Ai, J., ... & Xu, T. (2020). Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. PLoS pathogens, 16(8), e1008705.
Javalkote, V. S., Kancharla, N., Bhadra, B., Shukla, M., Soni, B., Sapre, A., ... & Dasgupta, S. (2022). CRISPR-based assays for rapid detection of SARS-CoV-2. Methods, 203, 594-603.
Kim, S. E., Park, S. H., Park, W. J., Kim, G., Kim, S. Y., Won, H., ... & Lee, J. A. (2023). Evaluation of immunogenicity-induced DNA vaccines against different SARS-CoV-2 variants. Plos one, 18(12), e0295594.
Kotta, S., Aldawsari, HM, Badr-Eldin, SM, Alhakamy, NA, Md, S., Nair, AB, dkk. (2020b). Menjelajahi potensi titik karbon untuk memerangi COVID-19.
Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, dkk. Karakterisasi genomik dan epidemiologi epidemiologi tahun 2019. virus corona baru: implikasi untuk asal usul virus dan pengikatan reseptor. The Lancet. 2020; 395: 565–74. https://doi.org/ 10.1016/S0140-6736(20)30251-8 9.
Maruggi, G., Zhang, C., Li, J., Ulmer, JB, dan Yu, D. (2019). mRNA sebagai teknologi transformatif untuk pengembangan vaksin guna mengendalikan penyakit menular. Mol. Ther. 27, 757–772. doi:10.1016/j.ymthe.2019.01.020.
Murray, K., Stahl, S., & Ashton-Rickardt, P. G. (1989). Genetic engineering applied to the development of vaccines. Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 324(1224), 461-476.
Syam, A. F., Zulfa, F. R., & Karuniawati, A. (2021). Manifestasi Klinis dan Diagnosis Covid-19. eJournal Kedokteran Indonesia, 8(3), 382771.
Wang, X., Rcheulishvili, N., Cai, J., Liu, C., Xie, F., Hu, X., ... & Wang, P. G. (2022). Development of DNA vaccine candidate against SARS-CoV-2. Viruses, 14(5), 1049.
WHO (2020). Draf lanskap kandidat vaksin COVID-19.
Xu, J., Ma, Y., Song, Z., Sun, W., Liu, Y., Shu, C., ... & Liang, Q. (2023). Evaluation of an automated CRISPR-based diagnostic tool for rapid detection of COVID-19. Heliyon, 9(2).
Downloads
Published
Issue
Section
License
Copyright (c) 2025 laili aurelia, Baiq Aisya Adinda Nabila, Prianggawe Prianggawe, Nisrina Hurul Ain, Hilma Putri Nulandari
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
