STUDI SEROLOGI PADA PASIEN SUSPEK DEMAM BERDARAH DI PUSKESMAS BULILI, KOTA PALU
DOI:
https://doi.org/10.31004/jkt.v6i1.42041Keywords:
dengue, NS1, gejala, virusAbstract
Demam berdarah merupakan penyakit menular yang disebabkan oleh virus dengue dengan angka kejadian yang berulang terutama di negara-negara berkembang. Keberhasilan dalam pengendalian penyakit ini belum tercapai dan demam berdarah telah berevolusi dari wabah epidemi yang bersiklus menjadi wabah yang tidak bersifat musiman. Kurangnya dasar ilmiah, hingga saat ini kasus Demam Berdarah Dengue (DBD) dapat menjadikan kasus-kasus dengan manifestasi hemoragik dan tingginya angka kematian. Dari segi presentasi klinis, infeksi virus dengue secara klinis mirip dengan banyak demam tropis akut lainnya dan pengujian laboratorium memiliki peran penting dalam diagnosis dini dan manajemen pasien. Penelitian ini bertujuan mengetahui gambaran gejala klinis suspek demam berdarah dengue dengan penegakan uji laboratorium menggunakan deteksi IgG, IgM dan NS1 berdasarkan jenis kelamin, yang nantinya dapat dipakai sebagai acuan untuk penatalaksanaan yang tepat untuk menghindari kasus DBD dengan manifestasi berat. Penelitian ini merupakan penelitian deskriptif analitik dengan desain cross sectional studi. Sampel yang digunakan adalah plasma darah dari 43 pasien dengan gejala klinis demam berdarah yang berobat jalan di Puskesmas Bulili pada bulan Februari-Juni 2024. Metode sampling yang digunakan adalah purposive sampling. Data dianalisis menggunakan uji chi-square. Empat puluh tiga sampel plasma dilakukan tes adanya IgG, IgM dan NS1. Hasil tes tersebut didapat 7% positif IgG, 2,3% positif IgM dan 23,25% positif NS1. Gejala klinis yang timbul pada uji laboratorium IgG, IgM dan NS1 tidak spesifik. Uji NS1 harus dilakukan untuk menegakkan diagnosis DBD. Tidak ada hubungan antara deteksi IgG, IgM dan NS1 dengan jenis kelamin dan gejala klinis yang timbul.References
Akinsulie, O. C., & Idris, I. (2024). Global re-emergence of dengue fever: The need for a rapid response and surveillance. The Microbe, 4, 100107. https://doi.org/10.1016/J.MICROB.2024.100107
Andreata-Santos, R., Pereira, S. S., Pereira, L. R., Félix, A. C., Romano, C. M., & Ferreira, L. C. de S. (2020). Specificity of NS1-based immunochromatographic tests for dengue virus with regard to the Zika virus protein. International Journal of Infectious Diseases, 95, 276–278. https://doi.org/10.1016/j.ijid.2020.04.012
de Souza, L. J., Bastos Pessanha, L., Carvalho Mansur, L., Assed de Souza, L., Barbosa Tâmega Ribeiro, M., do Vale da Silveira, M., & Damian Souto Filho, J. T. (2013). Comparison of clinical and laboratory characteristics between children and adults with dengue. Brazilian Journal of Infectious Diseases, 17(1), 27–31. https://doi.org/10.1016/j.bjid.2012.08.020
Dinas Kesehatan Provinsi Sulawesi Tengah. (2021). Profil Kesehatan Provinsi Sulawesi Tengah. http://dinkes.sultengprov.go.id
Flórez, J. E. S., Velasquez, K. M., Cardona, Á. M. S., Jaramillo, B. N. R., Díaz, Y. E. O., Cardona, L. S. G., & Naranjo, M. A. (2024). Clinical Manifestations of Dengue in Children and Adults in a Hyperendemic Region of Colombia. American Journal of Tropical Medicine and Hygiene, 110(5), 971–978. https://doi.org/10.4269/ajtmh.23-0717
Fujimoto, D. E., & Koifman, S. (2014). Clinical and laboratory characteristics of patients with dengue hemorrhagic fever manifestations and their transfusion profile. Revista Brasileira de Hematologia e Hemoterapia, 36(2), 115–120. https://doi.org/10.5581/1516-8484.20140027
Hunsperger, E. A., Sharp, T. M., Lalita, P., Tikomaidraubuta, K., Cardoso, Y. R., Naivalu, T., Khan, A. S., Marfel, M., Hancock, W. T., Tomashek, K. M., & Margolis, H. S. (2016). Use of a rapid test for diagnosis of dengue during suspected dengue outbreaks in resource-limited regions. Journal of Clinical Microbiology, 54(8), 2090–2095. https://doi.org/10.1128/JCM.00521-16
Lima, M. R. Q., Nunes, P. C., & Santos, F. B. Dos. (2022). Serological Diagnosis of Dengue. Methods in Molecular Biology, 2409, 173–196.
Minh Lam, H., Thi Phuong, H., Ha Thao Vy, N., Thi Le Thanh, N., Ngoc Dung, P., Thi Ngoc Muon, T., Van Vinh Chau, N., Rodríguez-Barraquer, I., T Cummings, D. A., Wills, B. A., Boni, M. F., Rabaa, M. A., & Clapham, H. E. (2019). Serological inference of past primary and secondary dengue infection: implications for vaccination. https://doi.org/10.1098/rsif.2019.0207
Raafat, N., Blacksell, S. D., & Maude, R. J. (2019a). A review of dengue diagnostics and implications for surveillance and control. In Transactions of the Royal Society of Tropical Medicine and Hygiene (Vol. 113, Issue 11, pp. 653–660). Oxford University Press. https://doi.org/10.1093/trstmh/trz068
Raafat, N., Blacksell, S. D., & Maude, R. J. (2019b). A review of dengue diagnostics and implications for surveillance and control. In Transactions of the Royal Society of Tropical Medicine and Hygiene (Vol. 113, Issue 11, pp. 653–660). Oxford University Press. https://doi.org/10.1093/trstmh/trz068
Rai A, Azad S, Nautiyal S, & Acharya S. (2019). Correlation between hematological and serological parameters in dengue patients-an analysis of 2022 cases. Original Research Article Pathology Update: Tropical Journal of Pathology & Microbiology Available Online at: Www.Medresearch.In, 547. https://doi.org/10.17511/jopm.2019.i8.07
Redoni, M., Yacoub, S., Rivino, L., Giacobbe, D. R., Luzzati, R., & Bella, S. (2020). Dengue: Status of current and under-development vaccines. Reviews in Medical Virology, 20(4).
T Patil, S., K.L, Dr. V., & N. G, Dr. K. (2019). A study of clinical manifestations and complications of dengue fever in medical college hospital. International Journal of Medical Research and Review, 7(3), 224–230. https://doi.org/10.17511/ijmrr.2019.i03.13
Wang, S. M., & Sekaran, S. D. (2010). Early diagnosis of dengue infection using a commercial dengue duo rapid test kit for the detection of NS1, IGM, and IGG. American Journal of Tropical Medicine and Hygiene, 83(3), 690–695. https://doi.org/10.4269/ajtmh.2010.10-0117
WHO. (2009). Dengue guidelines, for diagnosis, treatment, prevention and control. http://whqlibdoc.who.int/publications/2009/9789241547871_eng.pdf. Geneva: World Health Organization
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Devi Oktafiani, Intania Riska Putrie, Ryka Marina Walanda, Tri Setyawati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
