EFEK EKSTRAK ETANOL DAUN LEILEM (CLERODENDRUM MINAHASSAE) TERHADAP KADAR GLUKOS DARAH TIKUS PUTIH JANTAN
DOI:
https://doi.org/10.31004/jkt.v4i3.16829Keywords:
Daun Leilem (Clerodendrum minahassae), Kadar Glukosa Darah, Tikus Putih JantanAbstract
Diabetes melitus merupakan penyakit kronis global dengan total penderita yang melebihi 500 juta jiwa. Daun leilem adalah tanaman endemik Sulawesi Utara yang berasal dari genus Clerodendrum yang diyakini memiliki potensi antidiabetes. Tujuan penelitian ini untuk mengetahui efek ekstrak etanol daun leilem terhadap kadar glukosa darah dan berat badan tikus putih jantan. Ekstraksi daun leilem menggunakan metode maserasi dengan pelarut etanol 96%. Penelitian ini menggunakan metode pretest posttest control group design dengan 5 kelompok, yaitu kelompok I kontrol positif metformin, kelompok II kontrol negatif Na-CMC 0,5 %, kelompok III, IV, dan V adalah ekstrak dosis 100, 200, dan 400 mg/kgBB. Semua kelompok diinduksi dengan aloksan sebanyak 120 mg/kgBB. Hasil penelitian menunjukkan bahwa ekstrak daun leilem memiliki pengaruh bermakna (Asymp. Sig. (2-tailed) 0,001) terhadap kadar glukosa darah tikus dengan presentase terbaik pada kelompok V dosis ekstrak 400 mg/kgBB dan tidak memiliki pengaruh signifikan terhadap berat badan.References
Al-Ishaq, R.K., Abotaleb, M., Kubatka, P., Kajo, K., Busselberg, D. 2019. Flavonoids and Their Anti-Diabetic Effects: Cellular Mechanisms and Effects to Improve Blood Sugar Levels. Biomolecules. 9 (430): 1-35.
Ayudya, R. 2022. Perbandingan Dosis Air Sirih Hijau (Piper Betle L) Dalam Menurunkan Kadar Glukosa Darah Tikus Putih (Rattus Norvegicus L) Yang Diinduksi Aloksan. Skripsi. Medan: Universitas Muhammadiyah.
Balsells, M., Gracia, P.A., Sola, I., Roque, M., Gich, I., Corcoy, R. 2015. Glibenclamide, metformin, and insulin for the treatment of gestational diabetes: A systematic review and meta-analysis. BMJ. 350: 102.
Datu, O.S., Lebang, J.S., Suoth, E.J. 2022. Efek Pemberian Ekstrak Buah Salahk (Salacca zalacca) dalam Menurunkan Kadar Glukosa Darah Pada Tikus Model Diabetes Melitus. Jurnal MIPA. 12 (1): 30-33.
Deng, H., Jiacheng Z., Qihui, Y., Xiaohui, D., Shuang, Z., Weixing, L., Beiping, T., and Shuyan, C. 2023. Effects of Dietary Steroid Saponins on Growth Performance, Serum and Liver Glucose, Lipid Metabolism and Immune Molecules of Hybrid Groupers. Metabolites. 13 (305): 1-14
Dewi, S.R.P., Marlamsya, D.O., Bikarindrasari, R. 2017. Efek Antikaries Ekstrak Gambir Pada Tikus Jantan Galur Wistar. Majalah Kedokteran Gigi Indonesia. 3 (2): 83-92.
Dipiro, J.T., Wells, B.G., Schwinghammer, T.L., Dipiro, C.V. 2015. Pharmacotherapy Handbook Ninth Edition. UK: Mcgraw-Hill Education Companies.
Fitriana, M., Wawan, H., Khoerul, A., Triyasmono, L., Rahmanto, B., Andriani, S., Ainah, N. 2020. Karakteristik Fisika Sediaan Suspensi Ekstrak Etanol Daun Gaharu (Aquilaria Microcarpa Baill.) Dengan Variasi Carboxymethyl Cellulose Sodium (Cmc-Na). Jurnal Pharmascience. 7 (1): 125-131.
Fourny, N., Lan, C., Bernard, M., Desrois, M. 2021. Male and Female Rats Have Different Physiological Response to High-Fat High-Sucrose Diet but Similar Myocardial Sensitivity to Ischemia-Reperfusion Injury. Nutrients. 13 (9): 2914.
Hasima., Faridahb, D.N., Safithria, M., Husnawatia., Setiyonoc, A., Manshurd, H.A. 2020. Aktivitas Penurunan Kadar Glukosa pada Tikus yang Diinduksi Aloksan dari Ekstrak Air Angkak, Bekatul dan Kombinasinya. Journal of Agro-based Industry. 37 (2): 171-179.
Ighodaro, O. M., Adeosun, A. M., & Akinloye, O. A. (2017). Alloxan-Induced Diabetes, A Common Model For Evaluating The Glycemic-Control Potential Of Therapeutic Compounds And Plants Extracts In Experimental Studies. Medicina. 53 (6): 365–374.
International Diabetes Federation (IDF). 2021. International Diabetes Federation: Diabetic Atlas 10th Edition. Belgia.
Jie, W.P. 2018. Efektivitas Pelarut Etanol 96% dan Aquadest pada Ekstrak Jahe Merah Terhadap Jamur Candida albicans (In Vitro). Skripsi. Fakultas Kedokteran Gigi USU, Medan.
Kairupan, C.F., Mantiri, F.R., Rumende, R.R.H. 2019. Phytochemical Screening and Antioxidant Activity of Ethanol Extract of Leilem (Clerodendrum minahassae Teijsm. & Binn) as an Antihyperlipidemic and Antiatherosclerotic Agent. IOP Conf. Series: Earth and Environmental Science. 217: 1-7.
Kar, P., Goyal, A.K., Das, A.P., Sen, A. 2014. Antioxidant and Pharmaceutical Potential of Clerodendrum L.: An Overview. International Journal of Green Pharmacy. 8 (4): 210-216.
Kaul, K., Tarr, J.M., Ahmad, S.I., Kohner, E.M., Chibber, R. 2012. Introduction to Diabetes Mellitus Chapter I. United Kingdom: Landes Bioscience and Springer Science.
Khaerati, K., Amini, D., Ihwan. 2020. Aktivitas Antidiabetes Ekstrak Air-Etanol, n-Heksan, dan Etil Asetat Uwi Banggai (Dioscorea alata L.) Dengan Metode Induksi Aloksan Pada Mencit Jantan (Mus musculus) Jurnal Farmasi Galenika. 6 (2): 243-252.
Khamchan, A., Paseephol, T., Hanchang, W. 2018. Protective effect of wax apple (Syzygium samarangense (Blume) Merr & LM Perry) against streptozotocin-induced pancreatic ß- cell damage in diabetic rats. Biomed Pharmacother. 108: 634-645.
Kitukale, M.D., dan Chandewar, A.V. 2014. An Overview on Some Recent Herbs Having Antidiabetic Potential. Journal of Pharmaceutical, Biological, and Chemical Sciences. 5 (6): 190-6.
Lucchesi, A.N., Cassettari, L.L., and Spadella, C.T. 2015. Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans. Journal of Diabetes Research. 2015 (494578): 1-11
Malin, S.K., dan Kashyap, S.R. 2014. Effects of metformin on weight loss. Current Opinion in Endocrinology & Diabetes and Obesity. 21 (5): 323–329.
Nangoy, B.N., Queljoe, E.D., Yudistira, A. 2019. Uji Aktivitas Antidiabetes Dari Ekstrak Daun Sesewanua (Clerodendrum squamatum Vahl.) Terhadap Tikus Putih Jantan Galur Wistar (Rattus norvegicus L.). Pharmacon Jurnal Ilmiah Farmasi. 8 (4): 774-780.
Ngatidjan, 1991. Petunjuk Laboratorium: Metode Laboratorium Dalam Toksikologi. Yogyakarta: Fakultas Kedokteran Universitas Gadjah Mada.
Nuralifah, Fitrawan, L.O.M., Parawansah, Trisetya, M. 2022. Histopatologi Organ Pankreas Tikus DM Tipe 2 Yang Diberi Ekstrak Etanol Daun Gedi Merah (Abelmoscus manihot L. Medik). Jurnal Syifa Sciences and Clinical Research. 4 (1): 141-151.
Oliveira., Miranda, L.M., Agostini, C., Lima, W., Camini, C., Costa, C. 2020. Silymarin Attenuates Hepatic and Pancreatic Redox Imbalance Independent of Glycemic Regulation in the Alloxan-induced Diabetic Rat Model. Biomed Environ Sci. 33: 690-700.
Pongoh, A.F., Queljoe, E.D., Rontinsulu, H. 2020. Uji Antidiabetik Ekstrak Etanol Bunga Papaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Pharmacon Jurnal Ilmiah Farmasi. 9 (1): 160-169.
Puspadewi, L. 2019. Efek Esktrak Biji Pepaya (Carica papaya L.) terhadap Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) Galur Wistar yang diinduksi Aloksan. Skripsi. Surakarta: Fakultas Kedokteran Umum Universitas Muhhamadiyah Surakarta.
Riwanti, P., F. Izazih, dan Amaliyah. 2020. Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50, 70 dan 96% Sargassum polycystum dari Madura. Journal of Pharmaceutical Care Anwar Medika. 2(2): 82-95.
Rybnikova, E.A., Vetrovoi, O.V., Zenko, M.Y. 2018. Comparative Characterization of Rat Strains (Wistar, Wistar–Kyoto, Sprague Dawley, Long Evans, LT, SHR, BD-IX) by Their Behavior, Hormonal Level and Antioxidant Status. Journal of Evolutionary Biochemistry and Physiology. (54): 374-382.
Simorangkir, M., Sinaga, E., Pasaribu, R., Silaban, S. 2022. Antidiabetic Activity Of Leaf Extract Of Clerodenrum Fragrans Vent Willd In Rattus Novergicus Induced by Alloxan. J Bioteknol Biosains Indones. 9 (1): 119-125.
Tambun, R., Limbong, H.P., Pinem, C., Manurung, E. 2016. Pengaruh Ukuran Partikel, Waktu, dan Suhu Pada Ekstraksi Fenol Dari Lengkuas Merah. Jurnal Teknik Kimia USU. 5 (4): 53-56.
Wardani, C.C., dan Kusumaningrum, I.D. 2017. Pengaruh Konsentrasi Natrii Carboxymethylcellulosum (Cmc Na) Sebagai Suspending Agent Terhadap Stabilitas Fisik Pada Sediaan Suspensi Kloramfenikol. Pharmaceutical & Traditional Medicine. 1 (2): 84-89.
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