PENGARUH PENDIAMAN PLASMA SITRAT SELAMA 2 JAM PADA SUHU 25°C TERHADAP HASIL ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT)

Authors

  • Nisa Munawaroh Program Studi Sarjana Terapan Teknologi Laboratorium Medis Universitas Aisyiyah Yogyakarta
  • Wahid Syamsul Hadi Program Studi D-IV Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas ‘Aisyiyah Yogyakarta
  • Isnin Aulia Ulfah Mu’awanah Program Studi D-IV Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas ‘Aisyiyah Yogyakarta

DOI:

https://doi.org/10.31004/jkt.v5i3.32288

Keywords:

activated partial thromboplastin time, hemostasis, plasma sitrat

Abstract

Gangguan perdarahan merupakan kelainan perdarahan yang terjadi akibat ketidakmampuan pembuluh darah, trombosit, dan faktor pembekuan pada sistem hemostatik. Tujuannya adalah untuk mengetahui pengaruh pendiaman sampel plasma sitrat selama 2 jam pada suhu 25°C terhadap hasil APTT. Jenis penelitian ini adalah Pre-experimental dengan desain One-shot case study. Jumlah sampel penelitian ini yaitu sebanyak 15 sampel, yang dilakukan secara purposive sampling. Berdasarkan hasil penelitian didapatkan rata-rata nilai APTT pada plasma sitrat yang segera diperiksa adalah 34,08 detik, sedangkan rata-rata nilai APTT pada plasma sitrat yang diakukan pendiaman selama 2 jam pada suhu 25°C adalah 38,98 detik. Hasil uji statistik Independent Sample T-Test didapat nilai signifikansi sebesar 0,046. Hasil penelitian ini menunjukkan bahwa terdapat perbedaan hasil yang signifikan secara statistik antara sampel plasma sitrat yang segera diperiksa dan sampel plasma sitrat yang dilakukan pendiaman selama 2 jam pada suhu 25°C terhadap nilai APTT.

References

Anandani, G. M., & Parikh, S. B. (2018). Effect of Pre-Analytic Variables on Prothrombin Time and Activated Partial Thromboplastin Time. Journal of Clinical & Diagnostic Research, 12(7).1-5. https://doi.org/10.7860/JCDR/2018/32666.11719

Durachim, A., & Astuti, D. (2018). HEMOSTASIS. In Bahan Ajar Teknologi Laboratorium Medik (TLM). Jakarta: Kementerian Kesehatan Republik Indonesia

Geelani, S., Wani, G. S., Khan, S. P., Qadri, S. M., Rasool, J., Quadri, S. S., & Khan, F. P. (2018). Effect of storage time on prothrombin time and activated partial thromboplastin time: study at a tertiary care center in Kashmir valley. Int J Sci Rep, 4, 182-5. http://dx.doi.org/10.18203/issn.2454-2156.IntJSciRep20182729

Kitchen, S., Adcock, D. M., Dauer, R., Kristoffersen, A. H., Lippi, G., Mackie, I., & Nair, S. (2021). International Council for Standardization in Haematology (ICSH) recommendations for processing of blood samples for coagulation testing. International journal of laboratory hematology, 43(6), 1272-1283.

Lippi, G., & Favaloro, E. J. (2017). Preanalytical issues in hemostasis and thrombosis testing. Methods in Molecular Biology, 1646, 29–42. https://doi.org/10.1007/978-1-4939-7196-1_2

Magnette, A., Chatelain, M., Chatelain, B., Ten Cate, H., & Mullier, F. (2016). Pre-analytical issues in the haemostasis laboratory: Guidance for the clinical laboratories. Thrombosis Journal, 14(1), 1–14. https://doi.org/10.1186/s12959-016-0123-z

Nafitri, A. (2020). Pengaruh Lama Penundaan Darah Sitrat Pada Suhu 20±1oc Terhadap Pemeriksaan Activated Partial Thromboplastin Time (APTT). Skripsi. Yogyakarta: Program Studi Diploma IV Jurusan Teknologi Laboraotrium Medis Poltekkes Kemenkes Yogyakarta.

Naim, N., & Baharuddin. (2014). Pengaruh Lama Penyimpanan Plasma Sitrat Terhadap Penetapan Activated Parsial Thromboplastin Time (APTT). Jurnal Media Analis Kesehatan, 5(2), 47–52.

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Published

2024-09-05

How to Cite

Munawaroh, N., Hadi, W. S. ., & Mu’awanah, I. A. U. . (2024). PENGARUH PENDIAMAN PLASMA SITRAT SELAMA 2 JAM PADA SUHU 25°C TERHADAP HASIL ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT). Jurnal Kesehatan Tambusai, 5(3), 8240–8246. https://doi.org/10.31004/jkt.v5i3.32288

Issue

Vol. 5 No. 3 (2024): SEPTEMBER 2024

Section

Articles

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Copyright (c) 2024 Nisa Munawaroh, Wahid Syamsul Hadi

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